The inclusion of exogenous phytase promotes phytate hydrolysis, releasing previously unavailable phosphorus and other associated nutrients. This improves nutrient utilization and reduces the need for inorganic phosphorus supplementation.

However, phytase efficacy depends on several factors, including dietary phytate concentration and supplemental calcium levels. Excess calcium may form complexes with phytate and inhibit phytase activity, reducing phosphorus release efficiency.

Therefore, optimizing the interaction between calcium, phosphorus, and phytase is critical for improving both productive efficiency and environmental sustainability in broiler production.

Experimental design

The study was conducted at the Nial experimental farm (bonÀrea Agrupa, Guissona) using 2,857 Ross 308 broilers from day one to 36 days of age. Birds were housed in 24 pens under controlled environmental conditions.

Researchers used a 2 × 2 factorial design comparing:

• High vs. low calcium and phosphorus levels (based on Aviagen 2014 vs. 2022 recommendations)
• High vs. low phytase inclusion (2,000 vs. 1,500 FYT/kg)

Birds were fed four feeding phases: pre-starter, starter, grower, and finisher diets.

Improved productive performance with lower Ca and P

After 36 days, broilers receiving diets with lower calcium and phosphorus levels showed significantly higher final body weight and average daily gain, together with improved feed conversion ratio compared with birds receiving higher mineral levels.

Regarding slaughter performance, birds receiving lower Ca and P diets tended to present:

• Higher carcass weight
• Lower carcass condemnation percentage

At the same time, diets containing the higher phytase concentration tended to improve carcass yield.

Bone mineralization remained unaffected

One of the most important findings was that reducing dietary calcium and phosphorus did not negatively affect bone health. Tibia ash content, used as an indicator of bone mineralization, was not influenced by either mineral level or phytase dose. :contentReference[oaicite:9]{index=9}

These results suggest that properly balanced diets may allow lower mineral inclusion without compromising skeletal integrity.

Reduced mineral excretion and environmental impact

As expected, fecal calcium and phosphorus excretion were significantly higher in birds fed the high-mineral diets. :contentReference[oaicite:10]{index=10}

Additionally, phosphorus excretion was higher in birds receiving the lower phytase concentration, suggesting reduced phytate phosphorus utilization and lower phosphorus release.

Practical implications for poultry nutrition

The findings reinforce the importance of revisiting traditional mineral recommendations in modern broiler nutrition. Advances in genetics, ingredient quality, enzyme technology, and precision nutrition may allow producers to formulate diets with lower calcium and phosphorus inclusion while maintaining or even improving productive performance.

At the same time, optimized phytase inclusion can contribute to:

• Improved phosphorus utilization
• Lower feed costs
• Reduced mineral excretion
• Better sustainability
• Enhanced carcass performance

Conclusion

The study demonstrated that moderate reductions in dietary calcium and phosphorus improved productive and slaughter performance without negatively affecting bone mineralization. Furthermore, higher phytase inclusion tended to improve carcass parameters while helping reduce phosphorus excretion.